FIG. 3.

Solubility of overproduced B. japonicum RpoH proteins. The supernatant (Sup) and pellet (Pellet) fraction of crude cell extracts prepared before (−) or after (+) IPTG induction were separated by centrifugation at 15,000 rpm (SS-34 rotor) at 4°C for 30 min. Control cells contain the vector pET24b(+) without insert. RpoH_1His, RpoH_2His, and RpoH_3His were produced from plasmids pRJ5086, pRJ5101, and pRJ5102, respectively. After electrophoresis in an SDS–12% polyacrylamide gel, the proteins were stained with Coomassie blue. The apparent molecular masses (in kilodaltons) of marker proteins are indicated to the left of the gel. Arrows point to the overproduced RpoH_His protein.