FIG. 5.
Transcription of the E. coli groESL promoter by E. coli RNA polymerase core enzyme and B. japonicum RpoH factors. (A) The enzyme combinations tested are indicated. Core, E. coli RNA polymerase core enzyme; RpoH, purified RpoHHis protein; Ec holo, E. coli RNA polymerase holoenzyme; Bj holo, B. japonicum RNA polymerase holoenzyme. (B) Primer extension analysis of in vitro-synthesized RNA. The enzyme with which the transcript was generated is indicated. The primer extension and sequencing reactions (TCGA) were performed with oligonucleotide Sig123. The transcription start site is depicted by an arrow.