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. 2023 Aug 7;79(1):183–197. doi: 10.1097/HEP.0000000000000547

FIGURE 3.

FIGURE 3

Host genetics determines infection frequency and viral dynamics in the liver. (A) NrHV viral RNA in situ hybridization in liver tissue sections from mock or NrHV-infected C57BL/6J, CC046, CC071, or CC080 mice 2, 7, 28, or 272 dpi. Nuclei are stained blue, and viral RNA is labeled brown. (B) Percent cells NrHV RNA positive using RNAscope in situ hybridization quantified using Definiens Architect. C57BL/6J Time (N): Mock (6), 2 dpi (4), 7 dpi (4), 28 dpi (5), and 272 dpi (7). CC046 Time (N): Mock (7), 2 dpi (4), 7 dpi (3), 28 dpi (6), and 272 dpi (5). CC071 Time (N): Mock (7), 2 dpi (4), 7 dpi (2), 28 dpi (5), and 272 dpi (15). CC080 Time (N): Mock (6), 2 dpi (4), 7 dpi (3), 28 dpi (4), and 272 dpi (8). (C) NrHV genome copy number in 500ng total liver RNA by qRT-PCR. C57BL/6J Time (N): Mock (4), 2dpi (4), 7 dpi (4), 28 dpi (5), and 272 dpi (7). CC046 Time (N): Mock (4), 2dpi (4), 7 dpi (3), 28 dpi (6), and 272 dpi (5). CC071 Time (N): Mock (4), 2 dpi (4), 7 dpi (2), 28 dpi (5), and 272 dpi (15). CC080 Time (N): Mock (4), 2 dpi (4), 7 dpi (3), 28 dpi (4), and 272 dpi (8). Asterisks in B and C indicate statistical significance by 2-Way ANOVA Tukey’s multiple comparisons test. Abbreviations: CC, Collaborative Cross; NrHV, Norway rat hepacivirus.