Fig. 5.
Effect of sevoflurane on NK cell-mediated cytotoxicity assessed by flow cytometry. Results were analyzed using Kruskal-Wallis tests with post-hoc Conover comparisons. Variables are presented as the median with the first and third quartiles (n = 4 per group). Target cancer cells (T; MCF-7, MDA-MB-453, and HCC-70) were stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) and co-cultured with NK92-MI cells (effector cells [E]) at a 1:1 ratio (e.g., E:T = 1 × 105 : 1 × 105) or 10:1 (e.g., E:T = 1 × 106 : 1 × 105). PI: propidium iodide, C: control group, S6: sevoflurane 600 µM group, S12: sevoflurane 1200 µM group. *P < 0.05 compared to C.