FIG. 5.

Restriction maps of the chromosomal fragments cloned in this work and sequence of the ompR234 mutation. The ompR region of PHL628 was cloned in vivo on pR′7 (see text). A 7.8-kb BamHI fragment and a 1.285-kb SmaI-EcoRI fragment conferring the adherent phenotype were successively subcloned into pBR325 (to generate plasmids pOV711 and pOV737). Part of the nucleotide sequence of ompR (32) is also shown. The single-base pair mutation is indicated by a black square, with the corresponding modification of the amino acid sequence given in the solid box. The mutation created a NaeI restriction site (see text).