TABLE 1.
E. coli K-12 strains and plasmids used
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| β63 | MG1655 malA-kan mutT | P. Marlière |
| CT110 | CSH57 thiA(pULB110) | A. Toussaint |
| HB101 | F− Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacYI proA2 rpsL20 xyl-5 mtl-1 recA13 Δ(mrcC-mrr) | Laboratory collection |
| MC4100 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | Laboratory collection |
| MG1655 | λ− F− prototroph | Laboratory collection |
| MH225 | MC4100 Φ(ompC-lacZ+)10–25 | 10 |
| MH513 | MC4100 Φ(ompF-lacZ+)16–13 | 10 |
| PHL628 | MG1655 malA-kan ompR234a | This study |
| PHL644 | MC4100 malA-kan ompR234 | This study |
| PHL645 | MC4100 malA-kan | This study |
| PHL690 | MG1655 malA-kan | This study |
| PHL691 | HB101(pR′7) | This study |
| PHL744 | MC4100 malT54::Tn10 ompR234 | This study |
| PHL745 | MC4100 malT54::Tn10 ompR234 csgA::kan | This study |
| PHL804 | MC4100 csgA::kan | This study |
| PHL806 | PHL804(pCSG4) | This study |
| PHL839 | MH513 malA-kan ompR234 | This study |
| PHL840 | MH225 malA-kan ompR234 | This study |
| PHL841 | MH513 malA-kan | This study |
| PHL842 | MH225 malA-kan | This study |
| PHL874 | PHL804(pOV874) | This study |
| PHL875 | PHL745(pCSG4) | This study |
| PHL876 | PHL745(pOV874) | This study |
| PHL893 | TK821 csgA::kan(pCSG4) | This study |
| PHL894 | TK821 csgA::kan(pOV874) | This study |
| RL101 | β63 ompR234 | This study |
| RL102 to -111 | β63 adr-102 to adr-111 | This study |
| TK821 | MC4100 ompR331::Tn10 | 10 |
| TST3 | F−araD139 Δ(argF-lac)169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR malT54::Tn10 | CGSC 6139 |
| YMel-1 | YMel csgA::kan | 21 |
| Plasmids | ||
| pAT003 | pBR322 with a 7.8-kb BamHI fragment harboring the ompB operon | 19 |
| pBR325 | Apr Tcr Cmr | Laboratory collection |
| pCSG4 | pUC19 with a 3.5-kb HindIII fragment containing the csgBA operon | 21 |
| pOV874 | pCSG4 carrying a uidA-kan fusion gene inserted at the unique ClaI site of the csgA gene | This study |
| pN496 | pBluescript Cmr containing a uidA-kan gene fusion | 13 |
| pOV711 | pBR325 with a 7.8-kb BamHI fragment harboring the ompR234 mutation | This study |
| pOV737 | pBR325 with a 1.285-kb EcoRI-SmaI fragment harboring the ompR234 mutation | This study |
| pR′7 | pULB110 derivative harboring the malA::kan marker and the ompR234 mutation of PHL628 | This study |
| pULB110 | RP4::Mu3A, Apr Tcr Kms | A. Toussaint |
a
ompR234 is equivalent to adr-101 (see text).