FIG. 2.

Phenotypes of one representative M. smegmatis INH^r T^s mutant, the ndh-4 mutant, and complementation with the ndh and mdh genes. The ndh⁺ parent strain and the ndh-4 mutant shown here carry the E. coli-mycobacterial shuttle plasmid pMV261, which confers Km^R. The lower half of each plate shows the ndh-4 mutant complemented by the ndh gene of M. tuberculosis (left; pYUB805) and the mdh gene of M. bovis BCG (right; pYUB808); these genes are expressed from the groEL promoter of plasmid pMV261. The plate in panel D was incubated at 42°C for 4 days, all other plates were incubated 6 days at 30°C. The concentration of both INH and ETH was 50 μg/ml, and all plates contained kanamycin.