TABLE 4.
Complementation of the ndh-4 mutant by minimal plasmids carrying the ndh or mdh genea
| Strain | Relevant genotype | Thermosensitivity | INH susceptibility (MIC, μg/ml) | ETH susceptibility (inhibitory zone diam ± SD) | Supplement required |
|---|---|---|---|---|---|
| mc21421 | ndh+/pMV261 | Tr | 5 | 3.5 ± 0.3 | None |
| mc21422 | ndh+/pMV261::ndh (M. tuberculosis) | Tr | 5 | 3.4 ± 0.1 | None |
| mc21423 | ndh+/pMV261::mdh (M. bovis BCG) | Tr | 1 | 4.6 ± 0.1 | None |
| mc21424 | ndh-4/pMV261 | Ts lethal | >100 | 0 | CAA |
| mc21425 | ndh-4/pMV261::ndh (M. tuberculosis) | Tr | 2.5 | 3.3 ± 0.3 | None |
| mc21426 | ndh-4/pMV261::mdh (M. tuberculosis complex) | Tr | 1 | 5.8 ± 0.1 | None |
a
All strains were derived from the ndh+ parent strain mc2155. Plasmid pYUB805 is pMV261::ndh (M. tuberculosis); plasmid pYUB808 is pMV261::mdh (M. tuberculosis complex). See footnotes to Table 3 for explanations of data categories.