Table 2.

Mutations targeted by Novaplex™ SARS-CoV-2 variants VII assay and three versions of PMA MassARRAY panels.

Method	Mutation	Control
Novaplex™ SARS-CoV-2 variants VII assay	S gene: HV69del, E484A, N501Y	RdRp gene
MassARRAY® ABDO V1a	S gene: S13I, T20N, A67V, HV69del, D80A, Y144del, W152C, F157L, R190S, LLA241del, D253G, V367F, K417N, K417T, L452R, E484Q, E484K, N501Y, A570D, Q613H, D614G, H655Y, Q677H, P681R, P681H, A701V, T716I, F888L, S982A, T1027I, E1092K, H1101Y, V1176F, D1118H	N gene: C29208del
MassARRAY® Omicron V1a	S gene: T19R, T19I, A67V, HV69del, T95I, GVY142del, N211del, G339D, S373P, S375F, K417N, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, T574K, D614G, H655Y, P681R, P681H, D796Y, N856K, Q954H, N969K, L981F, D1118H
MassARRAY® Omicron V2a	S gene: T19R, T19I, A67V, HV69del, T95I, GVY142del, N211del, G339D, R346K, S373P, S375F, K417N, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, T547K, D614G, H655Y, P681R, P681H, D796Y, N856K, Q954H, N969K, L981F, D1118H, I1221T
MassARRAY® Omicron V3a	S gene: V3G, T19I, T19R, A67V, HV69del, T95I, G142D, K147E, G339H, G339D, R346K, K417N, L452R, N460K, S477N, T478K, E484A, F486V, Q493R, Q498R, N501Y, Y505H, T547K, D614G, H655Y, P681H, P681R, D796Y, N856K, Q954H, L981F, I1221T
	N gene: P151S, ORF7b: L11F

^aDifferent mutation panels were used at different time periods as the assay was continually improved. ABDO V1 was used until 16 December 2021. Omicron V1 was used from 18 December 2021 until 3 January 2022. Omicron V2 was used from 4 January 2022 until 28 March 2022. Omicron V3 was used from 27 March 2022 onwards.