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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Thromb Haemost. 2022 Dec 6;123(4):380–392. doi: 10.1055/a-1993-4193

Fig. 3. Incorporation of monodansyl cadaverine (MDC) into α2 -antiplasmin, fibronectin and actin using FXIII-A* and FXIII-A°.

Fig. 3

The addition of 100 nM FXIII-A* or FXIII-A° initiated the incorporation of MDC (1 mM) into α2 -antiplasmin and actin (2 μM). For fibronectin (1 μM), 200 nM FXIII-A*/FXIII-A° were used. The positive control used was MDC crosslinked into N, N-dimethylated β-casein. The quantification of the gels was performed as described in the methods section. The curves represent one phase exponential association fits of the data as a function of time. Data were reported as mean ± SD (N=3). The gels for the MDC incorporation and the quantification curves include α2 -antiplasmin (A and B), fibronectin (C and D), and actin (E and F). t-tests analysis showed that activity differences between FXIII-A* and FXIII-A° are significant (***P < 0.001) for actin and not for (P > 0.05) α2 -antiplasmin and fibronectin.