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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Thromb Haemost. 2022 Dec 6;123(4):380–392. doi: 10.1055/a-1993-4193

Fig. 5. Transglutaminase activities between FXIII-A* and FXIII-A° on Fbg. αC Q237 are significantly different.

Fig. 5

FXIII-catalyzed Q237-GEE crosslinking assays between (A) WT Fbg αC (233 – 425, black) and the FXIII binding site variants (B) E396A (green) and (C) 389 Stop (blue) were performed using 50 nM of either FXIII-A* (circles, solid lines), or FXIII-A° (triangles, dashed lines). Reactions were monitored by measuring the concentration of uncrosslinked Q237 remaining at each time point. Plots represent one phase exponential decay fits of uncrosslinked Q237 concentrations as a function of time. Data were reported as mean ± SD (N = 3). (D) FXIII GEE-crosslinking activity after 15 minutes of reaction time was compared between Fbg. αC WT, E396A, and 389 Stop. Shown here is the mean uncrosslinked Q237 concentration ± SD in μM after 15 minutes of reaction time (N = 3). Statistical significance was determined using the Student’s t-test (*P < 0.05, **P < 0.01).