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Western analysis of RNA polymerase α subunit expression. Samples were whole-cell extracts of bacterial suspensions normalized by optical density to contain the same numbers of cells. The blot was probed with monoclonal antibody 4RA2, which recognizes an epitope on the α subunit conserved between E. coli and B. pertussis, kindly provided by Nancy Thompson and Richard Burgess. Sizes are indicated in kilodaltons. Lanes: a, BP953; b, BP1187; c, BP1196; d, BP1246.
