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. 2023 Aug 16;214(3):260–274. doi: 10.1093/cei/uxad099

Figure 1.

Figure 1.

The expression of IL-36γ and IL-38 in HDM-induced chronic asthmatic mice. (A) The protocol of chronic asthma animal experimental model (n = 6 per group). (B and C) Representative histopathological images and quantification of the lung tissues in mice were performed with H&E staining. Scale bar = 50 μm (×200). (D) The total and differential inflammatory cell counts in BALF were determined by Giemsa staining. (E and F) Representative histopathological images and quantification of the lung tissues in mice were performed with Masson staining. Scale bar = 50 μm (×200). (G) The HYP level in lung of each group mice was measured by HYP assay kit. (H and I) Representative immunoreactivity images and quantification of IL-36γ staining in lung tissues from each group. (J) The mRNA level and (K) concentration of IL-36γ in lung tissue and BALF in each group mice. (L) The mRNA level and (M) concentration of IL-38 in lung tissue in each group mice. Bar diagrams and data are presented as the mean ± standard deviation (SD). * vs. PBS. * P < 0.05; **P < 0.01; ***P < 0.001