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. 2023 Dec 14;13:22193. doi: 10.1038/s41598-023-49594-0

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Charts showing length (A) and area (B) of the NADPH-d positive megaloneurites in the sacral spinal cord of aged dogs (2000 megaloneurites counted). (C) Histogram of diameter distribution of NADPH-d positive fibers. (D) The diameter of the megaloneurites (500 NADPH-d megaloneurites counted) and normal fibers (500 fibers counted). The k of unit label in (B) represents 1000, and asterisks in horizontal bars indicate statistically significant comparisons (***p < 0.001).