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. 2023 Dec 14;13:22193. doi: 10.1038/s41598-023-49594-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The Megaloneurites in horizontal sections in the DGC in the sacral segment of aged dogs. (A) Horizontal sections in the low power microphotograph confirmed that the megaloneurites are organized in a regular interval vertical to the rostrocaudal axis. (B) is the magnification of (A). (C), (D) High power microphotographs demonstrated a significant difference between the megaloneurites and normal fibers and neurons. Open arrowheads: NADPH-d neurons, black arrows: normal NADPH-d neurites, black arrowheads: abnormal megaloneurites. Scale bar in (A) = 200 μm, in (B) = 100 μm, in (C), (D) = 50 μm.