Northern hybridization analysis of ben gene transcripts. Regions of benA and benE were labeled to make probes A and E, respectively, as described in Materials and Methods. Arrows indicate transcripts detected in RNA from the wild-type ADP1 grown with benzoate (A) or from ACN32, with a transcriptional terminator following the benA::lacZ fusion, grown with benzoate and cis,cis-muconate (B). Total wild-type RNA in one sample (A, lane 1) was stained with methylene blue to demonstrate the positions of the rRNA species.