TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid(s) | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Acinetobacter strains | ||
| ADP1 | Wild type (BD413) | 14 |
| ADP205 | Δ(catM-catB3205) | 20 |
| ISA13 | catM::ΩS4013 | 30 |
| ISA25 | Δ(catBCIJF4025) | 7 |
| ISA29 | Δ(benE-catA4029) | 7 |
| ISA35 | Δ(benMABC4025)catM::ΩS4013 | 30 |
| ISA36 | benM::ΩS4036 | This study |
| ACN9 | benM::ΩK5008 catM::ΩS4013 | This study |
| ACN32 | benA::lacZ-Kmr5032 | This study |
| ACN39 | benA::lacZ-Kmr5032 Δ(catBCIJF4025) | This study |
| ACN41 | benA::lacZ-Kmr5032 Δ(benE-catA4029) | This study |
| ACN42 | benA::lacZ-Kmr5032 benM::ΩS4036 Δ(catM-catB3205) | This study |
| ACN47 | benA::lacZ-Kmr5032 benM::ΩS4036 | This study |
| ACN146 | benM::ΩS4036, point mutation T→A (Fig. 2) | This study |
| ACN147 | benM::ΩS4036, point mutation G→A (Fig. 2) | This study |
| ACN149 | benM::ΩS4036, point mutation G→A (Fig. 2) | This study |
| ACN157 | benA::lacZ-Kmr5032 benM::ΩS4036, point mutation T→A (Fig. 2) | This study |
| ACN158 | benA::lacZ-Kmr5032 benM::ΩS4036, point mutation G→A (Fig. 2) | This study |
| ACN160 | benA::lacZ-Kmr5032 benM::ΩS4036, point mutation G→A (Fig. 2) | This study |
| Plasmids | ||
| pUC19 | Apr, cloning vector | 37 |
| pRK415 | Tcr, cloning vector | 16 |
| pKOK6 | Source of promoterless lacZ-Kmr cassette | 18 |
| pHP45 | Source of ΩS | 29 |
| pUI1637 | Source of ΩK | 6 |
| pIB1351, pIB1352 | benM to -D (E1 to E2; Fig. 2) in pUC19 | 24 |
| pIB1354 | benA to -D (H to E2; Fig. 2) in pUC19 | 24 |
| pIGG5 | Part of benM (E1 to S; Fig. 2) in pUC19 | This study |
| pIGG16 | Part of benK, benCDE (E1 to K1, K2 to RV2; Fig. 2) in pRK415 | This study |
| pIGG23 | Part of benM (E1 to K1; Fig. 2) in pUC19 | This study |
| pIGG24 | Part of benM (E1 to H; Fig. 2) in pUC19 | This study |
| pIGG26 | Part of benM (S to E2; Fig. 2) in pUC19 | This study |
| pIGG27 | ΩS in benM SalI site (S; Fig. 2) of pIGG24 | This study |
| pBAC12 | ΩK in benM SalI site (S; Fig. 2) of pIGG24 | This study |
| pBAC14 | All of benM (E1 to P; Fig. 2) in pRK415 | This study |
| pBAC54 | lacZ-Kmr in benA NsiI site (N; Fig. 2) of pIB1354 | This study |
| pBAC64 | Part of benM (RV1 to K1; Fig. 2) in pUC19 | This study |
a
Kmr, kanamycin resistant; Apr, ampicillin resistant; Tcr, tetracycline resistant.