FIG. 2.

The spectral changes during the tautomerization of 2-hydroxymuconate and the deamination of 2-aminomuconate. (A) Tautomerization of 2-hydroxymuconate (296 nm) to 4-oxalocrotonate (236 nm) in 67 min, initiated by the addition of an ethanolic solution of 2-hydroxymuconate to potassium phosphate buffer (25 mM, pH 8.0, 0.15 M NaCl). (B) Deamination of 2-aminomuconate (326 nm) in the presence of the deaminase (0.0056 mg of protein/ml) in 35 s.