TABLE 1.

Purification of 2-aminomuconate deaminase

Step	Total protein (mg)	Total activity (μmol · min−1)	Sp act (μmol · min−1 · mg−1)	Purification (fold)	Yield (%)
Cell extract	420	72.4	0.17	1	100
DEAE-Sepharose	42.8	84.6	1.98	11.6	117
Hitrap Cu(II)-chelating	25.2	69.5	2.76	16.2	96
Sephacryl S-300	12.4	59.2	4.77	28.1	82
Hitrap-Q	3.7	42.6	11.5	67.6	59
Phenyl-Sepharose CL-4B	0.69	26.1	37.8	222	36