Table 2.
Effects of curcumin on As3+-induced ROS formation in rat hepatocytes
| Incubation time with curcumin | Addition | |||
|---|---|---|---|---|
| 60 min | 30 min | 15 min | ||
| 97±8 | 65±7 | 56±9 | Control rat hepatocytes | |
| 279±13a | 266±8a | 255±11a | +As3+ (50 µM) | |
| 217±21b | 295±13b | 277±19 | + Curcumin (10 µM) | |
| 254±19 | 248±16 | 239±14 | + Curcumin (5 µM) | |
| 168±12b | 142±7b | 116±8b | + Curcumin (2 µM) | |
Hepatocytes (106 cells/ml) were incubated in Krebs–Henseleit buffer (pH 7.4) at 37◦C for 1 hr following the addition of As3+ at EC50, 2hr. DCF formation was expressed as fluorescent intensity units. Values are expressed as mean±SD of three separate experiments (n=3).a Significant difference in comparison with the control hepatocytes (p<0.05).b Significant difference in comparison with the As3+-treated hepatocytes (p<0.05).