. 2023 Nov-Dec;13(6):641–650. doi: 10.22038/AJP.2023.22634

Table 4.

Effects of curcumin on As³⁺-induced mitochondrial membrane potential loss in rat hepatocytes

Incubation time			Addition
9±2	5±3	2±1	Control rat hepatocytes
42±3^a	21±4^a	11±3^a	+As³⁺ (50 µM)
54±5^b	29±3^b	12±3	+ Curcumin (10 µM)
36±5	19±4	6±3	+ Curcumin (5 µM)
18±5^b	7±3^b	3±2^b	+ Curcumin (2 µM)

Hepatocytes (10⁶ cells/ml) were incubated in Krebs–Henseleit buffer pH 7.4 at 37^◦C for 1.0 hr following the addition of EC_{50, 2hr}of As³⁺. Mitochondrial membrane potential was determined as the difference in mitochondrial uptake of the rhodamine 123 between the control and treated hepatocytes and expressed as fluorescence intensity unit (10). Values are expressed as mean±SD of three separate experiments (n=3).^a Significant difference in comparison with the control hepatocytes (p<0.05).^b Significant difference in comparison with the As³⁺ treated hepatocytes (p<0.05).