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. Author manuscript; available in PMC: 2023 Dec 14.
Published in final edited form as: J Am Chem Soc. 2023 May 15;145(20):11097–11109. doi: 10.1021/jacs.3c00598

Figure 1.

Figure 1.

BDHI electrophile reacts with proteins in the human proteome. (A) Covalent inactivation mechanism of acivicin for glutamine amidotransferase. (B) Structures of fluorescent-BDHI probes. (C) In-gel fluorescence scanning of recombinant KEAP1, TG2, and ALDH1A1 labeled with BDHI-TAMRA (2) and BDHI-PEG4-TAMRA (3) probes (1 μM, 2 h). (D) In-gel fluorescence image depicting protein bands labeled with fluorescent-BDHI probes in the human proteome. Soluble proteome from Jurkat cells was treated with the indicated probes for 2 h, followed by SDS-PAGE and in-gel fluorescence scanning.