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. 2023 Nov 30;10:1287312. doi: 10.3389/fnut.2023.1287312

Figure 3.

Figure 3

Gene expression analysis of THP-1 macrophage-like cells transfected with plant-derived miR482f mimic. (A) mRNA levels of putative targets NFAM1 and CLEC7A. (B) Gene expression levels (mRNA) of pro-inflammatory (TNF, IL1B, and TLR6) and anti-inflammatory (IL10) biomarkers and BCL2L12. THP-1 macrophage-like cells were transfected with 60 nM of miRVana mimic miR482f (5′-UCUUUCCUACUCCACCCAUUCC-3′) and a scramble sequence as a negative control. Gene expression levels were measured at 48 h of transfection by qPCR. Results are the gene expression levels of each gene normalized to the housekeeping gene TBP and calculated by the 2−ΔΔCt method, establishing comparisons with the negative control. NC, negative control. The results are presented as mean percentage ± standard error of the mean (SEM) (n = 2 independent experiments, run in triplicate; NC: n = 5; miR482f: n = 6). p-value: * p < 0.05, ** p < 0.01, *** p < 0.001, t = 0.0708.