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Quantitative PCR using chemically un-modified DNA primer probes (a) strategy to use light-up DNA-aptamer pair for quantitation in PCR. A red sphere indicates the dye, the blue part of the primer is complementary to the template, and the red part is the dye-specific aptamer (b) DNA aptamer and its partner dye can be used to monitor the progress of a PCR reaction with a decrease in fluorescence. Data represents the mean and standard deviation of n = 3 replicates.
