Figure 2.

qPCR using light-up dye-aptamer and conventional DNA intercalating dye-based method with Taq polymerase and its quantification using Ct values. (a) Agarose gel electrophoresis to determine the limit of detection using the custom designed primers. (b) Exponential increase in fluorescence with the progress of the PCR reaction using Taq polymerase and DNA intercalating dye, TB Green, n = 3 (c) Ct values were calculated from (b) and plotted against the log₁₀ (copies/μL). The curve was fitted to a linear model and the fitted equation with the R² value is shown. Each curve represents the mean and standard deviation of the replicates. (d) Exponential decrease in fluorescence with the progress of the PCR reaction using Taq polymerase with DIR dye and DIR2-1 aptamer fluorescence detection, n = 3. (e) Ct values were calculated from (d) and plotted against the log₁₀ (copies/μL). The curve was fitted to a linear model and the fitted equation with the R² value is shown. Each curve represents the mean and standard deviation of the replicates.