Figure 3.

Determination of the concentration of an unknown DNA sample with qPCR using conventional and light-up dye-aptamer detection chemistry. (a) Exponential decrease in DIR dye and DIR2-1 aptamer fluorescence with the progress of the PCR reaction using Taq polymerase. (b) Ct values were calculated from (a) and plotted against the log₁₀ (DNA amount). The curve was fitted to a linear model and the fitted equation with the R² value is shown. Each curves represents the mean and standard deviation of n = 4 replicates. (c) Exponential increase in fluorescence with the progress of the PCR reaction using Taq polymerase and DNA intercalating dye TB green, n = 4. (d) Ct values were calculated from (c) and plotted against the log₁₀ (DNA amount). The curve was fitted to a linear model and the fitted equation with the R² value is shown. Each curves represents the mean and standard deviation of n = 4 replicates.