Table 1. Primers Used in the qPCR Amplification of hasB (GenBank: CP065056.1) and rpoB (GenBank: L27989.1).
target | primers (42 nt DIR2-1, bold) | nucleotides |
---|---|---|
hasB | 5′-atgggctcacaggaggctgag | 203647–203667 |
hasB | 5′-gacgacgacgctaggaaggcgttggtgggcacgccggtcgtc cctttggcaggcaatagccgc | 203834–203854 |
rpoB | 5′-tcacgtgacagaccgccg | 2461–2444 |
rpoB | 5′-gacgacgacgctaggaaggcgttggtgggcacgccggtcgtc ccagctgagccaattcatggg | 2366–2386 |