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. 2023 Nov 28;8(49):47277–47282. doi: 10.1021/acsomega.3c07599

Table 1. Primers Used in the qPCR Amplification of hasB (GenBank: CP065056.1) and rpoB (GenBank: L27989.1).

target primers (42 nt DIR2-1, bold) nucleotides
hasB 5′-atgggctcacaggaggctgag 203647–203667
hasB 5′-gacgacgacgctaggaaggcgttggtgggcacgccggtcgtc cctttggcaggcaatagccgc 203834–203854
rpoB 5′-tcacgtgacagaccgccg 2461–2444
rpoB 5′-gacgacgacgctaggaaggcgttggtgggcacgccggtcgtc ccagctgagccaattcatggg 2366–2386