FIG. 4.
(A) β-Galactosidase activity of the cwlF-lacZ transcriptional fusion strain 327FD. Open symbols indicate growth; closed symbols indicate β-galactosidase activity. t0 is defined as the onset of sporulation. (B) Northern blot analysis with a cwlF RNA probe. Each lane contained 10 μg of total RNA isolated from B. subtilis AC327 at t−1, t0, t1, t2, t3, t4.5, and t6 (lanes −1 to 6, respectively). The 23S and 16S rRNAs are 2.8 and 1.4 kb, respectively. The calculated size of the detected transcript is shown on the left.