FIG. 1.
Kinetics of asparagine uptake in JGY51 (gap1 gnp1) and JGY52 (gap1 gnp1 agp1). The rate of [14C]asparagine uptake was determined in JGY51 (filled symbols) and JGY52 (open symbols) as described previously (20, 26). The data was plotted as Michaelis-Menten (A) and Eadie-Hofstee (B) graphs. The Michaelis-Menten graphs were fitted to curves assuming two permeases (JGY51) and one permease (JGY52) with the Solver program of Microsoft Excel. Each data point is the average of at least three measurements, with a standard error of <10%. The deduced Kmapp for asparagine of Agp1 is 0.29 mM, and the Vmax is 9.0 nmol/min/mg (dry weight).