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. 2023 Nov 30;14:1301653. doi: 10.3389/fmicb.2023.1301653

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Copyright © 2023 Ding, Huang, Li, Tang, Li, Feng, Zhao, Zhang, Yuan, Shan and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity and sensitivity of the RT-LAMP assay. (A) Specificity of the RT-LAMP assay. The plus sign (+) and minus sign (−) represent positive samples and negative controls, respectively. Each reaction was performed with three repeats, and one representative readout is shown in the figure. (B) Sensitivity of the RT-LAMP assay. Serial dilutions of DTMUV RNA from 10⁹ copies/μL to 1 copy/μL were used in this assay. Each dilution was performed with three repeats, and one representative readout is shown in the figure.