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. 2023 Nov 30;14:1301653. doi: 10.3389/fmicb.2023.1301653

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Copyright © 2023 Ding, Huang, Li, Tang, Li, Feng, Zhao, Zhang, Yuan, Shan and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR-Cas12a-mediated cleavage assay. (A–C) crRNA-1, crRNA-2 and crRNA-3 targeting the C gene of DTMUV were used for the CRISPR-Cas12a-mediated cleavage assay. Each crRNA was performed with three repeats in this assay. (D) The fluorescence intensity value of each reaction was measured by a microplate reader. All data were analyzed with GraphPad Prism 5.0 (La Jolla, CA, United States). Significant differences were evaluated using a two-way analysis of variance (ANOVA). A p-value <0.05 was considered statistically significant. Statistical significance is indicated in the figure as follows: ^**p < 0.01 and ^***p < 0.001.