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. 2023 Nov 30;14:1301653. doi: 10.3389/fmicb.2023.1301653

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Copyright © 2023 Ding, Huang, Li, Tang, Li, Feng, Zhao, Zhang, Yuan, Shan and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization for cleavage time of the CRISPR-Cas12a-mediated assay. (A–F) Cleavage results of the CRISPR-Cas12a-mediated assay at different times. The reaction was performed with three repeats at each cleavage time. (G) The fluorescence intensity value of each reaction was measured by a microplate reader. All data were analyzed with GraphPad Prism 5.0 (La Jolla, CA, United States). Significant differences were evaluated using a two-way analysis of variance (ANOVA). A p-value <0.05 was considered to indicate a significant difference. Statistical significance is indicated in the figure as follows: ^**p < 0.01, ^***p < 0.001, and no significant difference is represented as “ns.”