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. 2023 Nov 30;14:1301653. doi: 10.3389/fmicb.2023.1301653

Figure 6.

Figure 6

Specificity and sensitivity of the RT-LAMP-based CRISPR-Cas12a assay. (A) Specificity of the RT-LAMP-based CRISPR-Cas12a assay. The plus sign (+) and minus sign (−) represent positive samples and negative controls, respectively. Each reaction was performed with three repeats, and one representative readout is shown in the figure. (B) Sensitivity of the RT-LAMP-based CRISPR-Cas12a assay. Serial dilutions of DTMUV RNA from 105 copies/μL to 1 copy/μL were used in this assay. Each reaction was performed with three repeats, and one representative readout is shown in the figure. (C) Sensitivity of SYBR Green qPCR. Tenfold serial dilutions of DTMUV RNA from 105 copies/μL to 1 copy/μL were used in this assay. Each reaction was performed with three repeats. A sample with an average Ct value ≤35 was positive, and a sample with an average Ct value >35 or undetectable DTMUV RNA level was negative.