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. 2023 Nov 30;14:1301653. doi: 10.3389/fmicb.2023.1301653

Figure 8.

Figure 8

Evaluation of the RT-LAMP-based CRISPR-Cas12a assay on tissue samples. (A) SYBR Green qPCR assay on tissue samples. Numbers 1–22 represent tissue sample identification numbers. Numbers 23 and 24 represent positive samples and negative controls, respectively. A sample with an average Ct value ≤35 was positive, and a sample with an average Ct value >35 or undetectable DTMUV RNA level was negative. (B) RT-LAMP-based CRISPR-Cas12a assay on tissue samples. Lanes 1–22 represent tissue samples. Lanes 23 and 24 represent the positive sample and negative control, respectively. In each figure, the red asterisk indicates the negative samples identified by the two assays.