Figure 2.

Intrachain hydrogen bonds (HBs) within mAb-bound peptides

For each crystallized Fab-peptide complex, the peptide sequence is shown with Fab-bound N-core and C-core motifs positioned as indicated and unresolved residues colored in gray. Filled circles and arrows represent HBs, noting acceptors and donors, respectively, with donor residues at the tail end of the arrow. Main chain donor/acceptor atoms are indicated by blue symbols, and side chain donor/acceptor atoms are depicted as red symbols. HBs mediated solely by main-chain atoms are represented by symbols above the peptide sequence, whereas side-chain-mediated HBs are shown below the sequence. 1210-like structures are shaded with a green background, MGG4-like structures are shaded orange, and Ky230 and Ky224 structures with distinct binding modes are shaded in pink and blue, respectively. The resolution of each Fab-peptide structure is indicated below the corresponding peptide. See also Table S3. X-ray crystallography data collection and refinement data for 1210-like mAbs and variants, related to Figures 2–5, Table S4. X-ray crystallography data collection and refinement data for MGG4-like mAbs and variants, related to Figures 2–4, Table S5. X-ray crystallography data collection and refinement data for Ky230 and Ky224, related to Figures 2 and 7.