TABLE 1.

Promoter constructs used in this study

Plasmid(s)	Description
pS16.2	Synthetic S16 promoter, representing promoter sequences from −42 to +5, cloned into pSD9
pS16.4	Synthetic S16 promoter, representing sequences from −42 to −17, cloned into pSD9
pS16.2(TG−)	pS16.2 with CC instead of TG 1 bp upstream of the −10 region of the promoter
pS16.2A to -F and pS16.2(TG−)A to −F	Contain various fragments (44 to 276 bp) derived from the multiple cloning region of plasmid pGEM5Zf(+) cloned into the SphI restriction site of pS16.2 [or pS16.2(TG−)], giving rise to six mosaic pS16.2 [or pS16.2(TG−)] constructs containing identical −10 but different −35 regions
pT125(TG−)	Original T125 promoter from pSD7.T125, which does not contain the TGN motif, cloned into pSD9
pT125(TG−).2	Construct containing the T125 promoter sequence till the −29 position and lacking the −10 region
pT125	Synthetic T125 promoter fragment, containing the sequence TG instead of GA 1 bp upstream of the −10 region sequence, cloned into pT125(TG−) as described in the text
pT125A to -F and pT125(TG−)A to −F	Contain various fragments (44 to 276 bp) derived from the multiple cloning region of plasmid pGEM5Zf(+) cloned into the ApaI restriction site of pT125 [or pT125(TG−)], giving rise to six mosaic pT125 [or pT125(TG−)] constructs containing identical −10 but different −35 regions