Fig 1.
Progression of disease and neurological signs as a function of inoculum size and inoculum preparation (frozen stock or freshly grown culture). (A) All rabbits were infected via intra-cisternal inoculation of Mtb HN878 and necropsied when they reached a neurological score of 4. Rabbits were scored on deviations from baseline for body and head position, balance, and limb function (Table 1). Sparklines are provided to the right of each row to visualize neurological deterioration versus time. (B) Evolution of body weight throughout disease progression and neurological deterioration in nine rabbits infected with 106 CFUs from a frozen stock of Mtb HN878 (other groups shown in Fig. S1). The time when each rabbit transited to a neurological score of 1 is indicated by enlarged red symbols.