. 2023 Nov 16;67(12):e00860-23. doi: 10.1128/aac.00860-23

TABLE 1.

Summary features of top 10 Canadian bla_KPC-encoding plasmid clusters among PLSDB

Primary cluster ID	No. Canadian plasmids	Median size (kb)	Predicted mobility^a^,b	Replicon type^a^,b	Relaxase type^a^,b	MPF type^a^,b	No. genera	Core genes (%)^d	bla_KPC allele^a	bla_KPC frequency	Tn4401 variant^a	Mean no. ARGs per plasmid^c	Plasmid taxonomic unit (PTU)^e
AA002	62	75	Conjugative	IncL/M	MOBP	MPF_I	12	0.09	bla_KPC-3	0.34	Tn4401b	5.1	L/M
AA007	31	57.3	Conjugative	IncN	MOBF	MPF_T	15	0.06	bla_KPC-3	0.38	Tn4401b	6.1	N1
AA013	10	43.6	Mobilizable	Rep_cluster_1367	MOBC	−^f	8	0.18	bla_KPC-2	0.86	Tn4401b	2.5	E8
AA014	13	55.6	Conjugative	IncN	MOBF	MPF_T	4	0.28	bla_KPC-3	0.71	Tn4401b	1.4	N2/3
AA017	7	47	Conjugative	IncN	MOBF	MPF_T	5	0.14	bla_KPC-2	0.18	Tn4401b	5	N2/3
AA029	9	113.6	Conjugative	IncFIB, IncFII, rep_cluster_2183	MOBF, MOBF	MPF_F	4	0.04	bla_KPC-2	0.86	Tn4401a	3.6	FK
AA042	21	16.5	Mobilizable	ColRNAI_rep_cluster_1857	MOBC	−	7	0.03	bla_KPC-3	1	Tn4401b	1.7	Could not be assigned
AA085		185.7	Conjugative	IncFIB, IncFII, rep_cluster_2183	MOBF, MOBF	MPF_F	1	0.06	bla_KPC-2	0.12	Tn4401a	5.9	FK
AA109	7	97.9	Non-mobilizable	Rep_cluster_2268 [novel repE(pEh60-7)]	−	MPF_F	1	0.2	bla_KPC-3	0.78	Tn4401b	1	Could not be assigned
AA124	10	48.8	Conjugative	IncX5	MOBP	MPF_T	9	0.13	bla_KPC-3	0.4	Tn4401b	1.6	New (putative) PTU

^{^a}

Values indicate the most common genotype in the cluster and may not apply to all plasmids in the cluster.

^{^b}

Values obtained from MOB-suite (24, 26). Mobility is assigned based on the presence of relaxase (mobilizable) and/or MPF proteins (conjugative) or absence of both (non-mobilizable).

^{^c}

ARGs = antimicrobial resistance genes.

^{^d}

Core genes represent the number of genes present in >95% of plasmids in the cluster, divided by the total number of non-redundant genes in the cluster.

^{^e}

PTU values obtained from COPLA (27) .

^{^f}

"-" indicates no relaxase or MPF protein detected.