TABLE 1.
Overview of transcriptome sequences for the three fornicates
| Organism | Kipferlia bialata | Trepomonas sp. NIES-1444 | Hexamita sp. NIES-1440 | |
|---|---|---|---|---|
| Predominant bacterial genera (top 3) contaminated in culture (percentage of sequences per genus against all bacterial sequences) | Pseudoalteromonas (100)b | Undibacterium (38.9), Chryseobacterium (20.0), Sulfurospirillum (12.5) | Cutibacterium (85.5), Ralstonia (2.3), Alcanivorax (1.1) | |
| No. of bases sequenced (Gb) | 27.6b | 29.1 | 7.6 | |
| No. of assembled transcripts | 20,294b | 76,247 | 48,794 | |
| No. of bases in assembled transcripts (kb) | 28,897b | 61,267 | 38,374 | |
| No. of annotated CDS regions | 10,283 | 37,247 | 24,666 | |
| GC content of CDS regions (%) | 56.9 | 43.1 | 45.9 | |
| N50 of CDS regions (bp) | 2,820 | 1,466 | 1,295 | |
| Average length of annotated proteins (aa) | 516.3 | 370.1 | 328.9 | |
| BUSCO completeness for annotated proteins (data set: eukaryote odb 10) | Completea | 33.4 (S:31.8, D:1.6) | 20.4 (S:11.4, D:9.0) | 15.3 (S:10.6, D:4.7) |
| Fragment | 12.2 | 7.5 | 9.0 | |
| Missing | 54.4 | 72.1 | 75.7 | |
| Reference | Accession no. | PRJDB5457 and DRR083618 | PRJDB15716 and DRR460931 | PRJDB15717 and DRR460932 |
| Publicationb | Reference (3) | – | – | |
a
D, duplicate; S, single.
b
Numbers updated from original report; –, none, this study.