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. 2023 Sep 26;109(12):3905–3918. doi: 10.1097/JS9.0000000000000727

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Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.

This is an open access article distributed under the Creative Commons Attribution-NoDerivatives License 4.0, which allows for redistribution, commercial and non-commercial, as long as it is passed along unchanged and in whole, with credit to the author. http://creativecommons.org/licenses/by-nd/4.0/

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(A) Quantification of residual dsDNA, summarized as ng dsDNA/mg dry extracellular matrix weight. Concentration of dsDNA is significantly (P<0.001) decreased in decellularized (DC) vaginal tissue and partial (DC) vaginal tissue with enzymatic degradation (no extraction) compared to native mesoderm and native ectoderm controls. (B) Quantification of residual RNA, summarized as ng RNA/mg dry extracellular matrix weight. Concentration of RNA is significantly (P<0.001) decreased in DC vaginal tissue and partial DC vaginal tissue with membrane disruption (no enzymes) compared to native mesoderm and ectoderm controls. The data represents the means of seven experiments with SD. (C) Concentration of dsDNA is significantly (P<0.001) decreased in decellularized full intact vaginal tissue rings from mesoderm (DC Mesoderm) and ectoderm (DC Ectoderm) sections and for decellularization with high chemical concentrations (High concentration DC). (D) Concentration of RNA is significantly (P<0.01) decreased in decellularized full intact vaginal tissue rings from mesoderm (DC Mesoderm) and ectoderm (DC Ectoderm) sections and for decellularization with high chemical concentrations (High concentration DC). Significance is depicted with *P<0.05, **P<0.01, and ***P<0.001.