Table 1.
DAR, conjugation efficiency, DAR0, DAR1, and DAR2 species of the generated ADCs. Samples (5 μg) were analyzed by HIC (214 nm) without addition of the reference mAb wt.
| ADC | DAR | Conjugation efficiency |
DAR0 species |
DAR1 species |
DAR2 species |
|---|---|---|---|---|---|
| mAb1DesignA-SLAE | 1.83 | 92% | 0% | 17% | 83% |
| mAb1DesignB-SLAE | 1.69 | 85% | 2% | 26% | 72% |
| mAb1DesignC-SLAE | 1.67 | 84% | 0% | 33% | 67% |
| mAb2DesignA-SLAE | 1.79 | 90% | 0% | 21% | 79% |
| mAb2DesignB-SLAE | 1.77 | 88% | 1% | 21% | 78% |
| mAb2DesignC-SLAE | 1.69 | 85% | 0% | 31% | 69% |
| mAb1DesignA-LLAE | 2.00 | 100% | 0% | 0% | 100% |
| mAb1DesignB-LLAE | 1.96 | 98% | 0% | 4% | 96% |
| mAb1DesignC-LLAE | 1.95 | 97% | 0% | 5% | 95% |
| mAb2DesignA-LLAE | 1.98 | 99% | 0% | 2% | 98% |
| mAb2DesignB-LLAE | 1.90 | 95% | 1% | 8% | 91% |
| mAb2DesignC-LLAE | 1.96 | 98% | 0% | 4% | 96% |