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. Author manuscript; available in PMC: 2024 Nov 6.
Published in final edited form as: Dev Cell. 2023 Oct 16;58(21):2206–2216.e5. doi: 10.1016/j.devcel.2023.09.005

Key resource table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Deposited Data
mouse ETS1 universal PBM data Wei et al.33 thebrain.bwh.harvard.edu/uniprobe/index.php
Ciona robusta genome Satou et al.69 N/A
LacZ_6hpf_1–3 Racioppi et al.42 GEO: GSE126691
Human reference genome NCBI build 38, GRCh38 Genome Reference Consortium http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/human
Human fetal heart putative enhancers predicted from H3K27ac ChIP and ATAC-seq Richter et al.43 Supplementary Table 11 in source
iPSC-cardiomyocyte putative enhancers predicted from ATAC- seq and H3K27ac ChIP Benaglio et al.44 GEO: GSE133833
Human fetal heart putative enhancers predicted from p300/CBP May et al.45 GEO: GSE32587
Sequencing data for MPRA This paper SRA: PRJNA999706
Experimental Models: Organisms/Strains
Ciona intestinalis type A (Ciona robusta) M-REP N/A
iPSC-CMs Frazer Lab UDID_10948
iPSC-CMs Frazer Lab UDID_13948
Oligonucleotides
Oligonucleotides for mutagenesis, see Table S2 This paper N/A
Recombinant DNA
Plasmid: foxf295-bpfog>H2B-mCherry Levine & Christiaen Labs N/A
Plasmid: foxf295-ets1mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets2mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets3mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets4mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets5mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets6mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets1optT2C-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets3optT2G-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets4optT2C-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets134opt-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets134optAbl-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets12345opt-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets12345optAbl-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf295-ets123456mut-bpfog>H2B-mCherry This paper N/A
Plasmid: foxf3kb>foxfcDNA This paper N/A
Plasmid: foxf3kb-ets1OptT2C>foxfcDNA This paper N/A
Plasmid: foxf3kb-ets3OptT2G>foxfcDNA This paper N/A
Plasmid: foxf3kb-ets4OptT2C>foxfcDNA This paper N/A
Plasmid: gata4-g9-scp>unc76-GFP This paper N/A
Plasmid: gata4-g9-SNVopt-scp>unc76-GFP This paper N/A
Plasmid: gata4-g9–4opt-scp>unc76-GFP This paper N/A
Plasmid: pTNT-ciETS1 This paper N/A
Software and Algorithms
ImageJ Schneider et al.76 https://imagej.nih.gov/ij/
FastQC (version 0.11.2) N/A http://www.bioinformatics.babraham.ac.uk/projects/fastqc
Trim Galore (version 0.4.4) N/A http://www.bioinformatics.babraham.ac.uk/projects/trim_galore
Bowtie2 (version 2.3.2) Langmead et al.70 http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Samtools (version 1.2) Li et al.71 https://samtools.sourceforge.net
MACS2 (version 2.7.9) Zhang et al.72 N/A
BEDTools Quinlan et al.74 N/A
pyliftover 0.4 N/A https://pypi.org/project/pyliftover
Adobe Premiere Pro Adobe N/A
Adobe Animate Adobe N/A
Code to generate Fig 2A, 4A, 4C and putative Ciona developmental heart enhancers and putative human developmental heart enhancers This paper https://doi.org/10.5281/zenodo.8215976