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Hydrolysis of pNPP by the recombinant GST-PrpA fusion protein. The affinity-column-purified proteins (GST and GST-PrpA fusion) were incubated at 30°C in the presence of different ions. The hydrolysis of pNPP was followed by measurement of the change in OD410 (22). ⊡, GST-PrpA with 2 mM Mn2+; 
, GST with 2 mM Mn2+; 
, GST-PrpA with 2 mM Ca2+; and ▵, GST-PrpA with 2 mM Mg2+.
