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. 2023 Dec 15;133(24):e173160. doi: 10.1172/JCI173160

Figure 4. Endothelial knockdown of ATGL suppresses eNOS via the accumulation of LDs.

Figure 4

(A and B) WB of the indicated proteins (A) and quantification by qPCR of the indicated mRNAs (B) days 1–4 (D1–D4) after knockdown of ATGL by siRNA transfection in HUVECs. n = 3. (C) Schematic indicating the 2 approaches taken to reducing LD burden: enhancing lipolysis (with forskolin) or blocking TG synthesis (with siACSL or siDGAT1, see Supplemental Figure 10; or with DGAT inhibition). (D) WB of ATGL and eNOS in HUVECs treated for 2 days with siATGL, forskolin, or iDGAT1, as indicated. Quantification of eNOS protein levels relative to 14-3-3 is shown in right panel. n = 3. **P < 0.01, 1-way ANOVA.