Figure 8. Deletion of Adora2a suppresses the tumor development in a NE lung cancer model.

(A) A schematic showing the method of generation of a NE lung cancer model. Briefly, the Cre-expressing adenovirus (Adeno-Cre) were intratracheally injected into the Rb1^fl/fl; Trp53^fl/fl and Rb1^fl/fl; Trp53^fl/fl; Adora2a^fl/fl mice to establish the Adeno-DKO and Adeno-TKO lung cancer mouse models. (B) Body weight of Adeno-DKO (n = 8) and Adeno-TKO (n = 8) mice at early stage of 90 days after Adeno-Cre injection. (C) The H&E staining displays overall tumor formation of Adeno-DKO and Adeno-TKO mice in the lung at early stage; scale bar: 1 mm; zoom image scale bar: 50 μm. IHC images demonstrate the expression of SYP and Ki67 in the lung tumor of Adeno-DKO and Adeno-TKO mice, scale bar = 100 μm; zoom image scale bar: 30 μm. (D) The lung tissues of Adeno-DKO and Adeno-TKO mice at late stage of 240 days after Adeno-Cre administration are shown. Dotted red lines indicate the tumor area on the lung tissue. Scale bar: 2mm. (E) Quantification of lung tumor lesions with distinct diameters in Adeno-DKO (n = 4) and Adeno-TKO (n = 3) mice at late stage of 240 days after Adeno-Cre injection. (F) H&E images exhibit tumor formations in the lung of Adeno-DKO and Adeno-TKO mice at late stage of 240 days after Adeno-Cre injection. Scale bar: 1 mm. For statistical analysis, student’s t test was used for B. **P < 0.01, data are presented as mean ± SEM.