Skip to main content
. 2023 Dec 15;133(24):e171237. doi: 10.1172/JCI171237

Figure 4. Loss of podocyte Pfn1 activates the DNA damage response.

Figure 4

(A) Representative immunoblot images of cyclin D1, cyclin B1, P21, profilin1, and WT1 as loading control in control and Pfn1-KO mouse primary podocytes. (B) Quantification of immunoblots in A. n = 5 independent experiments. (C) Representative immunofluorescence image of P21 expression in control and Pfn1-KO mouse glomeruli at 5 weeks of age stained with P21 (green) and WT1 (red). Scale bar: 20 μm. (D) Quantification of immunofluorescence intensity of nuclear P21 per podocyte in C. Total of 310 podocytes in 5 mice. (E) Representative immunofluorescence images of P53 in control and Pfn1-KO mouse glomeruli at 5 weeks of age stained with P53 (green) and WT1 (red). Scale bar: 20 μm. (F) Quantification of immunofluorescence intensity of nuclear P53 per podocyte in E. Total of 310 podocytes in 5 different mice. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.