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. 2023 Dec 4;21(12):e3002417. doi: 10.1371/journal.pbio.3002417

Fig 9. FMRP regulates alternative splicing through redistribution of MBNL1 isoforms.

Fig 9

(A) Changes in alternative RNA splicing across categories. (B) Violin plots illustrating the distribution of delta PSI in Fmr1 and Mbnl1-depleted cells. The solid line is the median and the dashed lines are quartiles. P-value < 0.05, |delta PSI| > 0.05. (C) Venn diagram comparing RNAs exhibiting skipped exon events in Fmr1 and Mbnl1-depleted cells. (D) Correlation plot showing the relationship of delta PSI values between Fmr1 and Mbnl1-depleted cells. (E) Heatmap visualizing the PSI values of mis-spliced RNAs in Fmr1-depleted cells, rescued by the overexpression of nMBNL1. PSI values were adjusted by z-score. (F) Heatmap displaying the PSI values of mis-spliced RNAs in Fmr1-depleted cells and cytoplasmic MBNL1 expressing cells (siMbnl1+cMBNL1), which is similar to the splicing changes upon Fmr1-depletion. PSI values were adjusted by z-score. (G) PSI values of Slc30a4 exon 2, rescued by nMBNL1. P-values were calculated using rMATS (****p < 0.0001). At right, RT-PCR validation of Slc30a4 exon 2 splicing. Band intensity was quantified and mean ± SD is shown (ANOVA, **p < 0.01, ***p < 0.001). (H) PSI values presented for Carm1 exon 5. P-values were calculated using rMATS (****p < 0.0001). At right, RT-PCR validation of Carm1 exon 5 splicing. Band intensities were quantified and mean ± SD is shown (ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001). The underlying data can be found in S3 Data.