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. 2023 Dec 4;21(12):e3002409. doi: 10.1371/journal.pbio.3002409

Fig 5. Dubious ORF YIL059C encodes a signal peptide.

Fig 5

(A) Ribo-seq reads on canonical ORF YIL060W (top) and “dubious” ORF YIL059C (bottom). The bounds of each ORF are indicated in boxes. The location of the detected peptide is indicated in green. Reads are assigned to the reading frame in which the position they map to is the first position in a codon; on each strand, frame 1 corresponds to the reading frame of the ORF shown. (B) Probability of a signal peptide cleavage site across the YIL059C sequence, as predicted by TargetP [50]. The peptide detected in MS analysis is indicated by a green box. (C) Alignment of YIL059C with the highest identity protein matches at the homologous locus in Saccharomyces species. Only species with a homologous locus (at the DNA level) are shown. (D) Alignment of YIL060W, the canonical gene antisense to YIL059C, with its highest identity protein matches at the homologous locus in Saccharomyces species. The data underlying this Figure can be found in S1 Data.