Fig 6. Noncanonical protein YNL155C-A, detected by MS, is well translated and conserved in Saccharomyces genus.

(A) Predicted proteins and decoys detected in MS data at a range of expect score thresholds, among noncanonical proteins that could be encoded by ORFs that overlap canonical ORFs on alternative frames. (B) Ribo-seq reads across the YNL155C-A ORF. Reads are assigned to the reading frame in which the position they map to is the first position in a codon. Frame 1 is the reading frame of YNL155C-A. The full span of YNL155C-A and the start of YNL156C are shown. The position of the 2 peptides found in MS are in green. (C) Multiple sequence alignment of YNL155C-A with its homologs in the Saccharomyces genus. The data underlying this Figure can be found in S1 Data.