FIG. 5.
Deletion of pcm affects the development of the GASP phenotype. A culture of the competitor strain of interest (open symbols) which had been maintained in stationary phase for 10 days was mixed in a 1:1,000 ratio with a fresh overnight culture of JV1094 (Kmr parental strain; closed symbols). The competitor strains were as follows: (A) aged JV1090 (Tcr parental strain); (B) aged JV1098 (Tcr pcm mutant complemented by attB::pcm+); (C to E) aged JV1093 (Tcr pcm mutant). Each panel represents the result of a single experiment, representative of at least six experiments (A and B) or typical of the three different types of outcomes observed for the pcm mutants in eight experiments (C to E). Results similar to those shown in panels C and E were observed twice each, while results similar to those shown in panel D were observed four times.